Galloway, C., S. Dalvi, A. Shadforth, S. Suzuki, M. Wilson, D. Kuai, A. Hashim, L. MacDonald, D. Gamm, D. Harkin, and R. Singh. Characterization of Human IPSC-RPE on a Prosthetic Bruch’s Membrane Manufactured From Silk Fibroin. Invest Ophthalmol Vis Sci, 2018, p. PubMed Text.
Abstract
Purpose: RPE cell transplantation as a potential treatment for AMD has been extensively investigated; however, in AMD, ultrastructural damage affects both the RPE and its underlying matrix support, the Bruch’s membrane (BrM). An RPE monolayer supported by a surrogate scaffold could thus provide a more effective approach to cell-based therapy for AMD. Toward this goal, we aimed to establish a functional human induced pluripotent stem cell-derived (hiPSC)-RPE monolayer on a Bombyx mori silk fibroin (BMSF) scaffold.