Min, J., J. Phillips, W. Daly, W. Murphy, and D. Gamm. Evaluating the Effects of ECM and Elastic Modulus on Human Pluripotent Stem Cell-Derived Photoreceptor Enrichment. Investigative Ophthalmology & Visual Science, 2016, p. Epub Full Text.
Abstract
Purpose
Human pluripotent stem cell (hPSC)-based photoreceptor replacement strategies require efficient generation and isolation of photoreceptors, as well as the removal of proliferative retinal progenitor cells (RPCs). Extrinsic factors such as the extracellular matrix (ECM), as well as the stiffness (elastic modulus) of the extracellular environment are known to modulate cellular adherence, differentiation, and maturation. The purpose of this study was to determine the potential of these extrinsic factors to promote photoreceptor enrichment while reducing the number of RPCs.