Hardware-software co-design of an open-source automatic multimodal whole slide histopathology imaging system

Significance: Advanced digital control of microscopes and programmable data acquisition workflows have become increasingly important for improving the throughput and reproducibility of optical imaging experiments. Combinations of imaging modalities have enabled a more comprehensive understanding of tissue biology and tumor microenvironments in histopathological studies. However, insufficient imaging throughput and complicated workflows still limit the scalability of multimodal histopathology imaging.

Keywords: histopathology; multimodal imaging; neural networks; second harmonic generation; whole slide imaging; workflows.