Incorporation of hPSC-derived photoreceptors and RPE cells into microfabricated, honeycomb-patterned microscaffolds for outer retinal reconstruction

Madrigal, A., I. Lee, R. Xie, S. Min, J. Zhu, J. Jin, A. Ludwig, M. Phillips, S. Gong, Z. Ma, and D. Gamm. Incorporation of HPSC-Derived Photoreceptors and RPE Cells into Microfabricated, Honeycomb-Patterned Microscaffolds for Outer Retinal Reconstruction. Investigative Ophthalmology & Visual Science, 2023, p. Epub Full Text.

Abstract

Purpose

Blinding retinal diseases such as late-stage of age-related macular degeneration require the replacement of both retinal pigment epithelium (RPE) and photoreceptors (PRs). However, co-delivery of these cell types as an organized bilayer is technically challenging. To address this issue, we developed a 3D honeycomb (HC)-patterned, poly(glycerol sebacate) (PGS) micro-molded scaffold capable of organized reconstruction of the RPE-PR bilayer.