Gillette, A., R. DeStefanis, S. Pritzl, D. Deming, and M. Skala. Inhibition of B-Cell Lymphoma 2 Family Proteins Alters Optical Redox Ratio, Mitochondrial Polarization, and Cell Energetics Independent of Cell State. Journal of Biomedical Optics, 2022, pp. 056505-056505 Abstract
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Significance: The optical redox ratio (ORR) [autofluorescence intensity of the reduced form of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H)/flavin adenine dinucleotide (FAD)] provides a label-free method to quantify cellular metabolism. However, it is unclear whether changes in the ORR with B-cell lymphoma 2 (Bcl-2) family protein inhibition are due to metabolic stress alone or compromised cell viability. |