Smith, J., C. Liu, J. Degnan, J. Ouellette, M. Conklin, A. Kellner, C. Scribano, L. Hrycyniak, J. Oliner, C. Zahm, E. Wait, K. Eliceiri, and J. Rafter. Label-Free Fluorescence Lifetime Imaging for the Assessment of Cell Viability in Living Tumor Fragments. J Biomed Opt, 2024, p. Epub PubMed Text.
Abstract
Significance: To enable non-destructive longitudinal assessment of drug agents in intact tumor tissue without the use of disruptive probes, we have designed a label-free method to quantify the health of individual tumor cells in excised tumor tissue using multiphoton fluorescence lifetime imaging microscopy (MP-FLIM).
Keywords: apoptosis; cancer; cell death; fluorescence lifetime; fluorescence lifetime microscopy; metabolism; multiphoton; necroptosis; nicotinamide adenine dinucleotide phosphate.