Samimi, K., D. Desa, W. Lin, K. Weiss, J. Li, J. Huisken, V. Miskolci, A. Huttenlocher, J. Chacko, A. Velten, J. Rogers, K. Eliceiri, and M. Skala. Light-Sheet Autofluorescence Lifetime Imaging With a Single-Photon Avalanche Diode Array. J Biomed Opt, 2023, p. PubMed Text.
Abstract
Significance: Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a popular method to monitor single-cell metabolism within unperturbed, living 3D systems. However, FLIM of NAD(P)H has not been performed in a light-sheet geometry, which is advantageous for rapid imaging of cells within live 3D samples.
Keywords: autofluorescence; fluorescence lifetime imaging microscopy; light-sheet microscopy; nicotinamide adenine dinucleotide (phosphate); single-photon avalanche diode.