Sinha, D., G. Beeson, M. Wilson, J. Eells, C. Beeson, and D. Gamm. Utilization of an Extracellular Flux Assay to Measure Mitochondrial Respiration in HPSC-RPE. Investigative Ophthalmology & Visual Science, 2016, p. Epub Full Text.
Abstract
Purpose
Human pluripotent stem cell-derived RPE (hPSC-RPE) cells are being utilized to model retinal disease in vitro, as well as to potentially treat patients with certain blinding disorders via cell transplantation. In vivo, RPE cells form a highly metabolically active monolayer that performs several important functions requiring efficient mitochondrial oxidative metabolism. Thus, efforts to monitor and optimize hPSC-RPE health should at least in part take into account mitochondrial function. Toward this end, we examined the behavior of hPSC-RPE in a well-established, rigorous extracellular flux (XF) assay after optimizing critical assay parameters.