Credentials: Graduate Student, Department of Cellular and Molecular Pathology
BSc Cellular & Molecular Biology, Humboldt State University, Arcata, CA
Steven Mayerl is interested in using human pluripotent stem cells (hPSCs) to create more anatomically and functionally accurate models of retinal development and retinal diseases. The Gamm lab uses hPSCs to make 3-dimaensional optic vesicles (OVs), also known as retinal organoids. These
hPSC-OVs grow and differentiate over time to form all of the major cell classes found in the retina. However, over time the inner layers of the OV undergo cell death due to lack of metabolic support. The goal of his research is to study this phenomenon and develop methods to improve the health and the longevity of hPSC-OVs through various means, including the introduction of a perfusable microvasculature network similar to what is found in the inner retina in vivo.
Miyaoka, Yuichiro, Jennifer R. Berman, Samantha B. Cooper, Steven J. Mayerl, Amanda H. Chan, Bin Zhang, George A. Karlin-Neumann, and Bruce R. Conklin. “Systematic Quantification of HDR and NHEJ Reveals Effects of Locus, Nuclease, and Cell Type on Genome-Editing.” Scientific Reports 6 (March 31, 2016): 23549. doi:10.1038/srep23549.
Nguyen, Duy P., Yuichiro Miyaoka, Luke A. Gilbert, Steven J. Mayerl, Brian H. Lee, Jonathan S. Weissman, Bruce R. Conklin, and James A. Wells. “Ligand-Binding Domains of Nuclear Receptors Facilitate Tight Control of Split CRISPR Activity.” Nature Communications 7 (July 1, 2016): 12009. doi:10.1038/ncomms12009.
Yuichiro Miyaoka, Steven J. Mayerl, Amanda H. Chan, and Bruce R. Conklin. “Detection of HDR and NHEJ induced by genome editing at endogenous gene loci using Droplet Digital PCR.” in press in Springer, Methods in Molecular Biology: Digital PCR: Methods and Protocols.